The kit is for the quantitative level of Testosterone in the sample, adopt purified Bird Testosterone to coat microtiter
plate, make solid-phase antibody, then add Testosterone to wells, Combine Testosterone antibody with labeled HRP
to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and
the color change is measured at a wavelength of 450 nm. The concentration of Testosterone in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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