FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.

Purpose

This kit allows for the determination of IL-6 concentrations in Chicken serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Chicken IL-6 level in the sample，use Purified Chicken IL-6 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to wells, Combined IL-6 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-6   in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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