FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human α-MSH concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.3ng/ml-12 ng/ml
Sensitivity: 0.05ng/ml
Components:
| Assay plate (12 × 8 coated Microwells) |
1 |
| Standard:13.5ng/ml |
1×0.5ml |
| Standard Diluent |
1×1.5ml |
| HRP-Conjugate Reagent |
1×6ml |
| Sample Diluent |
1×6ml |
| Chromogen Solution A |
1×6ml |
| Chromogen Solution B |
1×6ml |
| Stop Solution |
1×6ml |
| Wash Solution |
1×20ml×30 fold |
| User manual |
1 |
| Adhesive Strip |
2 |
Product Principle:
The kit is for the quantitative level of α-MSH in the sample, adopt purified Human α-MSH antibody to coat microtiter plate,
make solid-phase antibody, then add α-MSH to wells, Combine α-MSH antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of α-MSH in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
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