Introduction

Sulfonamides (SAs3) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfonamides (SAs3) in honey, tissue, serum, milk, urine, and etc.

Principle of test

The Sulfonamides (SAs3) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. Take caloration with TMB substrate. The samples A value is an inverse relationship with SAs residue content. Lastly, compared with the standard curve can be concluded the SAs residues in sample.

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