Generic Name：Human anti-neutrophil cytoplasmic antibody,cANCA ELISA Kit

Purpose

This kit allows for the determination of cANCA concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human cANCA level in the sample，use Purified Human cANCA antibody to coat microtiter plate wells, make solid-phase antibody, then add cANCA to wells, Combined cANCA antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of cANCA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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