REAGEN^™ Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.

The unique features of the kit are:

• High recovery (70% - 95%), and cost-effective extraction methods for various samples.
• A quick ELISA assay (less than 1.5 hours regardless of number of samples).
•  High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The Nitroimidazole antibody has been coated in the plate wells. During the analysis, sample is added along with the Nitroimidazole -horseradish peroxidase (HRP) conjugate. If the target is present in the sample, it will compete for the Nitroimidazole antibody, thereby preventing the Nitroimidazole- HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of substrate((TMB), has an inverse relationship with the target concentration in the sample.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Meat/Liver/Kidney/Fish	0.25
Shrimp	0.25

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.