REAGEN^™ Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples.

The unique features of the kit are:

• High sensitivity and low detection limit (0.8 ppm)
• High reproducibility
• A rapid (30 minute), robust enzyme-based assay

Procedure Overview

REAGEN^™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.

Sensitivity (Detection Limit)

 

Sample Volume	Detection Limit (mg/L)
0.01 mL	8
0.1mL	0.8

 

The linear range of the assay is 0.8 – 1000 mg/L (ppm).

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.