Veratox for Casein Allergen is intended for the quantitative  analysis of food products,

such as juices,sauces and sorbets,or environmental surfaces for the presence of casein.

ASSAY PRINCIPLES

The Veratox for Casein Allergen test is a sandwich enzyme-linked  immunosorbenttion (Phosphate Buffered Saline, or PBS) by shaking  in a heated  water  bath. Extracted casein residue is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound residue is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound casein residue.After a second wash, the substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of allergen.

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.