REAGEN™ Melamine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of melamine in egg,milk, milk powder, infant formula, meat, fish, feed, chocolate yogurt, butter milk ,sour milk and other food samples. The unique features of the kit are:

• High recovery (>80%),rapid(10-40 minutes),and cost-effective extraction methods.
• High sensitivity(5ng/g or ppb)and low detection limit for liquid milk (0.2ppm).
• A quick ELISA assay(less than 2 hours regardless of number of samples).
• High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The enzyme-conjugate, will bind with the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Sensitivity (Detection Limit)

 

Sample Type	Detection Limit (g/g or ppb)
Egg	0.055ppm
Feed	0.5ppm
Meat /fish	0.055ppm
Milk	0.2ppm
Milk powder/infant formula	0.4ppm
Chocolate	0.4ppm
Yogurt/butter milk /sour milk	0.4ppm

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.