REAGEN^™Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. Compared with current commercially available kits,.

the unique features of the kit are:

• A quick spore growth assay (results can be read in about 3 hours).
• Detection of wide range of antibiotics in milk.
• High reproducibility.
• High sensitivity and low detection limit.

Procedure Overview

The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65°C, these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If milk samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged.

Sensitivity

 

Antibiotic	Detection Limit (ppb)	MRL (ppb)
Penicillin G	2	4
Ampicillin	3	4
Amoxicillin	4	4
Oxacillin	20	30
Cloxacillin	30	30
Gentamycin	30	100
Tylosin	50	50
Ceftiofur	50	100
Oxytetracycline	50	100
Tetracycline	50	100
Sulfamethoxazole	200	500

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.