REAGEN^™ Monensin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect monensin in feed to satisfy customer concerns about food safety. The Monensin ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost effective and easy to use. The features of the kit are:

• The extraction of monensin from samples can be finished in 45 -60 minutes with a high recovery rate of 75 - 105%

• High reproducibility
• A quick ELISA assay (less than 1.5 hours regardless of number of samples)

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The monensin antibody has been coated in the plate wells. During the analysis, sample is added along with the monensin-horseradish peroxidase conjugate. If monensin residue is present in the sample, it will compete for the monensin antibody, thereby preventing the monensin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the monensin residue concentration in the sample.

Sensitivity

 

Sample Type	Detection Limit (ng/g or ppb)
Feed	30

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.