REAGEN^™Domoic Acid (ASP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Domoic Acid in mussels, algae and water samples.

The unique features of the kit are:

• High recovery (>80%), rapid (10 - 30 minutes), and cost-effective extraction methods
• High sensitivity (0.125ppb) and low detection limit (30ppb for mussel samples and 6ppb for water samples)
• High reproducibility.
• A quick ELISA assay(less than 1.5 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Domoic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the Domoic Acid-horseradish peroxidase conjugate (Domoic Acid-HRP Conjugate) and Anti- Domoic Acid Antibody. If the Domoic Acid residue is present in the sample, it will compete with Domoic Acid-HRP Conjugate for the Anti-Domoic Acid Antibody, thereby preventing the Domoic Acid-HRP Conjugate from binding to the Anti-Domoic Acid Antibody. The Anti-Domoic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the domoic acid residue concentration in the sample.

Sensitivity

 

Sample Type	Detection Limit (ng/g or ppb)
Mussels	30
Water	6

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.