Product Description

REAGEN^™ Diethylstilbestrol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Diethylstilbestrol in feed, fish, meat, milk, milk powder, serum/plasma, shrimp, tissue and urine.

The unique features of the kit are:

• High recovery (80%-105%),rapid, cost-effective extraction methods.
• High sensitivity(0.15 ng/g or ppb) and low detection limit (meat/fish/shrimp 0.075 ppb and urine 0.25ppb).
• A quick ELISA assay (less than 2 hours regardless of number of samples).
• High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Meat/fish/Shrimp	0.075
Urine	0.25
Feed	0.15
Milk	0.2
Milk power	2
Serum/Plasma	0.75

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.