REAGEN^™Trimethoprim ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trimethoprim (TMP) in fish, shrimp, feed, meat (beef, chicken and pork), milk,serum and urine.

The unique features of the kit are:

• Rapid extraction method for various sample types
• High recovery rates (>80%)
• High sensitivity (0.015 ng/g or ppb)
• Quick ELISA assay (less than 2 hours regardless of number of samples)
• High reproducibility

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with Trimethoprim (TMP) antibody. During analysis, sample is added along with the TMP-horseradish peroxidase (TMP-HRP) conjugate. If the TMP residue is present in the sample, it will compete for the TMP antibody, thereby preventing the TMP-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the TMP residue concentration in the sample.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Feed	0.8
Fish/Shrimp/Meat/Liver/Kidney	0.2 (Method I); 10 (II)
Urine /Serum/ Plasma	0.2
Milk/milk powder	10

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.