The unique features of the kit are:

• High recovery(>75%), rapid (less than 60 minutes), and cost-effective extraction methods.
• High sensitivity (0.25 ng/g or ppb)
• High reproducibility.
• A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

REAGEN^™ Amantadine ELISA Test Kit is based on a competitive colorimetric ELISA assay. The plate wells are coated with anti-amantadine antibody. During analysis, sample is added along with the Amantadine-horseradish peroxidase (Amantadine-HRP) conjugate. If Amantadine residue is present in the sample, it will compete for the Amantadine antibody, thereby preventing the Amantadine-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the concentration of Amantadine residue in the sample.

Sensitivity

Sample type	Detection Limit (ng/g or ppb)
Muscle/ organization	0.25
egg	0.5

 

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.