REAGEN™Chloramphenicol (CAP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.

The unique features of the kit are:

• High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
• High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb) for shrimp, fish and meat samples.
• High reproducibility.
• A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The CAP antibody has been coated in the plate wells. During the analysis, sample is added along with the CAP-horseradish peroxidase conjugate. If the CAP residue is present in the sample, it will compete for the CAP antibody, thereby preventing the CAP-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the CAP residue concentration in the sample.

Sensitivity

 

Sample Type	Detection Limit (ng/g or ppb)
Feed	0.25
Fish/Shrimp/Meat	0.025
Eggs	0.05
Honey	0.05
Milk	0.25
Milk powder	0.1
Serum	0.04
Serum	0.5 (rapid extraction)
Urine	0.04
Urine	0.05 (rapid extraction)

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.