Hyroxine(T4), a form of thyroid hormones is the major hormone secreted by the follicular cells of the thyroid gland. Over 99% of thyroxine (T4) circulates in blood is bound to thyroxine-binding globulin (TBG). However, only the free (unbound) portion of Thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. Free thyroxine (T4) can be measured directly (FT4) or calculated as the free thyroxine index (FTI). The FTI tells how much free T4 is present compared to bound T4. The FTI can help tell if abnormal amounts of T4 are present because of abnormal amounts of thyroxine-binding globulin.

Sheep Free thyroxine (FT4) ELISA Kit employs a two-site sandwich ELISA to quantitate FT4 in samples. An antibody specific for FT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Sheep Free thyroxine (FT4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/sheep-free-thyroxine-ft4-elisa-kit-kte110073/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.