Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.Anderson S., Nature 290:457-465(1981).Horai S., Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).Maca-Meyer N., BMC Genet. 2:13-13(2001).

Sheep NADH-ubiquinone oxidoreductase chain 5 (MT-ND5) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND5 in samples. An antibody specific for MT-ND5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Sheep NADH-ubiquinone oxidoreductase chain 5 (MT-ND5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.