Phosphatidylglycerophosphate Synthase 1 belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is CDP-diacylglycerol:sn-glycerol-3-phosphate 3-phosphatidyltransferase. This enzyme participates in glycerophospholipid metabolism.Autoradiograms of replica prints, following enzyme assays, showed dark halos indicating the enzymatic synthesis of labeled phospholipid products. The method was also used to detect a cho 1 mutant defective in phosphatidylserine synthase and a strain that overproduces phosphatidylserine synthase. The method should become a valuable tool in isolating yeast strains defective in phospholipid biosynthetic enzyme activities and strains with overproduced enzyme activities.

Rat Phosphatidylglycerophosphate synthase 1 (PGS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGS1 in samples. An antibody specific for PGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Phosphatidylglycerophosphate synthase 1 (PGS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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