Oncostatin M(OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors.

Rat Oncostatin M (OSM) ELISA Kit employs a two-site sandwich ELISA to quantitate OSM in samples. An antibody specific for OSM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOSM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OSM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OSM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Oncostatin M (OSM) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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