Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes with each acting on either D-lactate (EC 1.1.2.4) or L-lactate (EC 1.1.2.3). The other two are NAD(P)- dependent enzymes with each acting on either D-lactate (EC 1.1.1.28) or L-lactate (EC 1.1.1.27). This article is about the NAD(P)- dependent L-lactate dehydrogenase. The five isozymes that are usually described in the literature each contain four subunits. The major isozymes of skeletal muscle and liver, M4, has four muscle (M) subunits; while H4 is the main isozymes for heart muscle in most species, containing four heart (H) subunits. The other variants contain both types of subunits.Usually LDH-2 is the predominant form in the serum. A LDH-1 level higher than the LDH-2 level , suggests myocardial infarction. The use of this phenomenon to diagnose infarction has been largely superseded by the use of Troponin I or T measurement.

Rat D-Lactate Dehydrogenase (D-LDH) ELISA Kit employs a two-site sandwich ELISA to quantitate D-LDH in samples. An antibody specific for D-LDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyD-LDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for D-LDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of D-LDH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat D-Lactate Dehydrogenase (D-LDH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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