Angiotensin II receptor, type 1 or AT1 receptor is an angiotensin receptor. It has vasopressor effects and regulates aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system.It is the best elucidated angiotensin receptor.The angiotensin receptor is activated by the vasoconstricting peptide angiotensin II. The activated receptor in turn couples to Gq/11 and thus activates phospholipase C and increases the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C. Activated receptor also inhibits adenylate cyclase and activates various tyrosine kinases.

Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate AT1R-Ab in samples. An antibody specific for AT1R-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAT1R-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AT1R-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AT1R-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/rat-angiotensin-ii-receptor-type-1-antibody-at1r-ab-elisa-kit-kte100918/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.