Formation of amyloid-beta is catalyzed by gamma-secretase, a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP) C-terminal fragment (APP-CTF) . The predicted full-length human GSAP protein contains 854 amino acids. Its sequence, particularly the C-terminal region, is highly conserved from chicken to human. Real-time PCR detected Gsap expression in diverse mouse tissues, with highest levels in lung, kidney, liver, and brain. Pulse-chain analysis of mouse N2a neuroblastoma cells showed that endogenous Gsap was synthesized as a 98-kD holoprotein that was rapidly processed into a 16-kD C-terminal fragment

Rat Gamma-Secretase Activating Protein (GSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate GSAP in samples. An antibody specific for GSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGSAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Gamma-Secretase Activating Protein (GSAP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.