MPG (N-Methylpurine DNA Glycosylase) is a Protein Coding gene. Among its related pathways are Recognition and association of DNA glycosylase with site containing an affected purine and Telomere C-strand (Lagging Strand) Synthesis. GO annotations related to this gene include damaged DNA binding and DNA-3-methyladenine glycosylase activity. Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Rat DNA-3-methyladenine glycosylase (MPG) ELISA Kit employs a two-site sandwich ELISA to quantitate MPG in samples. An antibody specific for MPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat DNA-3-methyladenine glycosylase (MPG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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