The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT) (E2); and dihydrolipoyl dehydrogenase (DLD) (E3). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex .Pyruvate dehydrogenase (E1) performs the first two reactions within the pyruvate dehydrogenase complex (PDC): a decarboxylation of substrate 1 (pyruvate) and a reductive acetylation of substrate 2 (lipoic acid).

Rat Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHA1 in samples. An antibody specific for PDHA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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