Peroxisome Proliferator-Activated Receptor gamma is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9-hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPAR-gamma. PPAR-gamma; is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts, and skeletal muscle. PPAR-γ plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three known family members are called PPAR alpha, delta, and gamma. Three N-terminal isoforms, called gamma1, gamma 2 and gamma 3, are known to arise by alternative splicing and promoter usage from the PPAR-gamma gene. RXR is an obligate partner for PPAR.

Rat Peroxisome proliferator-activated receptor gamma (PPARG) ELISA Kit employs a two-site sandwich ELISA to quantitate PPARG in samples. An antibody specific for PPARG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPARG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPARG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Peroxisome proliferator-activated receptor gamma (PPARG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.