Adenylate cyclase type 10 is a protein encoded by this gene belongs to a distinct class of mammalian adenylyl cyclase that is soluble in testis but particulate in sperm and other tissues. It is unlike other mammalian adenylyl cyclases because it is insensitive to G protein or forskolin regulation and is directly regulated by bicarbonate. It is localized in the cytoplasm and to organelles and is thought to function as a general bicarbonate sensor throughout the body. It plays an important role in the generation of cAMP in spermatozoa, playing possible roles in sperm capacitation, hypermotility, and/or the acrosome reaction.Genetic variations in this gene are associated with absorptive hypercalciuria type 2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Rat Adenylate cyclase type 10 (ADCY10) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY10 in samples. An antibody specific for ADCY10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Adenylate cyclase type 10 (ADCY10) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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