Surfactant protein D (SP-D) belongs to the collectin family.These proteins are oligomeric proteins composed of carbohydrate-recognition domains(CRD)attached to collagenous regions.Collectins are structurally similar to the ficolins although they make use of different CRD structures:C-type lectin domain for the collectins.The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type Ⅱcells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointestinal tract and in epithelial cells of various exocrine glands. SP-D is an important factor in the pulmonary anti-microbial defense. The anti-microbial defense mechanisms of SP-D are direct opsonization, neutralization and agglutination.

Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPD in samples. An antibody specific for SFTPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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