TRPM5 expression in several human and rodent cell lines, including murine neuronal cells, Burkitt lymphoma cells, murine B-lymphoma cells, HeLa cells, murine pancreatic beta cells, rat beta cells, and human pancreatic islets. No endogenous TRPM5 was detected in human embryonic kidney (HEK)-293 cells.Among the differentially expressed clones, one encoded Trpm5. In situ hybridization detected Trpm5 expression restricted to mouse taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, G protein gamma-13 subunit, phospholipase C-beta-2 (PLCB2), and inositol 1,4,5-trisphosphate receptor type III.

Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM4 in samples. An antibody specific for TRPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.