Proteoglycan 4 is an approximately 345-kD proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. The cDNA encodes a protein of 1,404 amino acids with a somatomedin B homology domain, heparin-binding domains, multiple mucin-like repeats, a hemopexin domain, and an aggregation domain. There are 3 consensus sequences for N-glycosylation and 1 chondroitin sulfate substitution site. The locus for autosomal recessive camptodactyly-arthropathy-coxa vara-pericarditis syndrome maps to chromosome 1q25-q31. This protein may act as a joint/intimal cell lubricant, and cell overgrowth may be primary to the pathogenesis of this protein.

Rabbit Proteoglycan 4 (PRG4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG4 in samples. An antibody specific for PRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rabbit Proteoglycan 4 (PRG4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.