Oxyntomodulin is a 37 amino acid peptide that contains the 29 amino acid sequence of glucagon followed by an 8 amino acid carboxyterminal extension. Radioimmunoassays using antisera that are not specific for both the C-and N-terminus of oxyntomodulin will also cross-react with glucagon, and possibly glicentin. Hence much of the earlier literature assessing circulating oxyntomodulin using non-specific assays may not be 100% accurate. Oxyntomodulin has also been shown to inhibit food intake following icv administration in rats. Injection of oxyntomodulin directly into the paraventricular nucleus also resulted in reduced food intake. Oxyntomodulin appears to inhibit food intake both prior to the onset of dark phase, as well as in fasted rats in the am. Remarkably, the anorectic actions of oxyntomodulin were blocked by the GLP-1 receptor antagonist exendin (9-39).

Pig Oxyntomodulin (OXM) ELISA Kit employs a two-site sandwich ELISA to quantitate OXM in samples. An antibody specific for OXM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOXM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OXM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OXM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Pig Oxyntomodulin (OXM) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.