L-lactate dehydrogenase A chain (LDH-A) catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene.

Mouse L-lactate dehydrogenase A chain (LDH-A) ELISA Kit employs a two-site sandwich ELISA to quantitate LDHA in samples. An antibody specific for LDHA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse L-lactate dehydrogenase A chain (LDH-A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.