The deduced 349-amino acid HIF1AN protein is homologous to rat Pass1 and to fly and worm sequences, indicating evolutionary conservation of this protein family. Binding analyses of truncated HIF1AN peptides showed that the inhibitory domain rather than the transactivating domains of HIF1A interacts with HIF1AN, although the presence of TAD-C optimizes binding.Functional analysis indicated that HIAF1AN inhibits HIF1A transcriptional activity in hypoxic and nonhypoxic cells. Further binding analysis determined that the N terminus of HIF1AN interacts with the N terminus of VHL, and that both interact with HIF1A at distinct sites (VHL with TAD-N of HIF1A), allowing the formation of ternary complexes of the 3 proteins.

Mouse Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) ELISA Kit employs a two-site sandwich ELISA to quantitate HIF1AN in samples. An antibody specific for HIF1AN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIF1AN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF1AN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF1AN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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