LAPTM4B was overexpressed in hepatocellular carcinoma compared with normal liver tissue, and its expression inversely correlated with the degree of tumor cell differentiation. Overexpression of full-length LAPTM4B increased the efficiency of colony formation in a hepatoma cell line.The deduced 317-amino acid protein has a calculated molecular mass of 35 kD. It has 4 transmembrane domains, an SH3 domain-binding PxxP motif at both its N and C termini, a conserved lysosome targeting signal at its C terminus, 1 potential N-glycosylation site, 8 putative phosphorylation sites, and 4 N-myristoylation sites. LAPTM4B shares 92% amino acid identity with its mouse homolog and 46% identity with human LAPTM4A.

Mouse Lysosomal-associated transmembrane protein 4B (LAPTM4B) ELISA Kit employs a two-site sandwich ELISA to quantitate LAPTM4B in samples. An antibody specific for LAPTM4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAPTM4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAPTM4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAPTM4B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Lysosomal-associated transmembrane protein 4B (LAPTM4B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.