The 11q23 gene involved in the translocations is MLL. In both patients, the translocation fused MLL to a gene that the authors designated AF10. The AF10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain that are highly homologous to similar domains in AF17 and BR140. Northern blot analysis showed that AF10 is expressed as a 5.5-kb mRNA in many tissues with highest expression in testis, and has an alternatively spliced exon.all known translocations involving MLL and AF10 or AF17 have involved breakpoints between the zinc finger and leucine zipper domains, and have yielded fusion proteins in which the MLL zinc fingers were replaced with leucine zipper regions.

Mouse Protein AF-10 (MLLT10) ELISA Kit employs a two-site sandwich ELISA to quantitate MLLT10 in samples. An antibody specific for MLLT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMLLT10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLLT10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLLT10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Protein AF-10 (MLLT10) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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