Binding immunoglobulin protein (BiP) is a molecular chaperone that uses ATP/ADP cycling to regulate protein folding by the protein disulfide isomerase (PDI) family of proteins. It is a 78kDa glucose-regulated heat shock protein and is involved in unfolded protein response. HSPA5 is its human gene. It binds to immunoglobulin heavy chain.When the nucleotide-binding domain of GRP78 interacts with ATP, its substrate-binding domain can interact with unfolded/misfolded protein. Subsequent ATP hydrolysis acts to strengthen the interaction between GRP78 and the unfolded/misfolded protein. Under these conditions PDI can then work to promote disulfide oxidation and rearrangement until the correct protein conformation is achieved.

Mouse Glucose Regulated Protein 78 (GRP78) ELISA Kit employs a two-site sandwich ELISA to quantitate GRP78 in samples. An antibody specific for GRP78 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRP78 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRP78 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRP78 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Glucose Regulated Protein 78 (GRP78) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.