By Northern blot analysis, Oh et al. (1996) found that mac25, or IGFBP7, wajs expressed as a 1.1-kb transcript in a broad spectrum of normal tissues. These authors reported that the predicted 277-amino acid protein contains a 26-amino acid signal sequence and the IGFBP motif (GCGCCXXC) at the N terminus. The IGFBP motif is located in a region containing a cluster of 12 conserved cysteines, of which 11 are found in IGFBP7. The amino acid sequences of IGFBP7 and IGFBPs share 20 to 25% identity. Oh et al. (1996) found that recombinant IGFBP7 specifically bound IGFs, although with relatively low affinity. Kim et al. (1997) proposed that IGFBP7, CTGF , NOV , and IGFBP10 constitute a subfamily of low-affinity IGFBPs.

Mouse Insulin-like growth factor-binding protein 7 (IGFBP7) ELISA Kit employs a two-site sandwich ELISA to quantitate IGFBP7 in samples. An antibody specific for IGFBP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIGFBP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGFBP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Insulin-like growth factor-binding protein 7 (IGFBP7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.