By means of yeast 1-hybrid screening of a liver cDNA library, Li et al. (1998) cloned a cDNA encoding a novel hepatocyte transcription factor, which they called HB1F for human B1-binding factor. The deduced 495-amino acid protein, which has a molecular mass of 54 kD, belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors and is closely related to steroidogenic factor-1 (SF1), another member of this subfamily.HB1F contains a DNA-binding domain with 2 zinc finger motifs, an FTZ-F1 box, and a ligand-binding domain. Northern blot analysis revealed that HB1F is expressed in liver, pancreas, and lung as a 5.2-kb transcript. An additional transcript of 3.8 kb was present in hepatoma cells HepG2. The authors identified 2 HB1F isoforms which differ in their A/B region.

Mouse Nuclear receptor subfamily 5 group A member 2 (NR5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate NR5A2 in samples. An antibody specific for NR5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNR5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Nuclear receptor subfamily 5 group A member 2 (NR5A2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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