DMWD contains 2 regions with significant homology to WD repeats. Northern blot analysis detected highest expression in mouse brain and testis, with significant expression in heart and lung. In situ hybridization of mouse embryos detected ubiquitous low expression in all tissues and enhanced expression in adult brain and testis. Dmwd expression began before embryonic day 9.5. From day 14.5 onward, Dmwd mRNA was detected in all neural tissues, especially in the telencephalon and mesencephalon. Later, expression was evident in distinct tubules of the mature testis and was restricted to secondary spermatocytes of stages VIII to XII of the spermatogenic proliferation cycle.Northern blot analysis of human and baboon RNA detected a 3-kb transcript.

Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) ELISA Kit employs a two-site sandwich ELISA to quantitate DMWD in samples. An antibody specific for DMWD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMWD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMWD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMWD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Dystrophia myotonica WD repeat-containing protein (DMWD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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