The enzyme encoded by G6pc is a multisubunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for glucose-6-phosphate, inorganic phosphate, and glucose. G6pc is one of three glucose-6-phosphatase catalytic-subunit-encoding genes in mouse. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in G6pc cause glycogen storage disease type I (GSD1).

Mouse Glucose-6-phosphatase (G6PC) ELISA Kit employs a two-site sandwich ELISA to quantitate G6PC in samples. An antibody specific for G6PC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyG6PC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G6PC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G6PC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Glucose-6-phosphatase (G6PC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-glucose-6-phosphatase-g6pc-elisa-kit-kte71216/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.