Keratin 9 is a type I cytokeratin. It is found only in the terminally differentiated epidermis of palms and soles. Mutations in the gene encoding this protein cause epidermolytic palmoplantar keratoderma.Langbein et al. (1993) reported the cloning and sequence of the KRT9 gene. The amino acid sequence showed the typical structure of type I cytokeratins, with a head (153 residues), alpha-helical coiled-coil-forming rod (306 residues), and tail (163 residues) domains. The keratin 9 protein displayed highest homology to human keratin 10 , not only in the highly conserved rod domain but also in large parts of the head and tail domains. Expression was confined to palmar and plantar epidermis. In situ hybridization and immunolocalization showed expression in subrabasal cell layers.

Mouse Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT9 in samples. An antibody specific for KRT9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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