Leukocyte cell-derived chemotaxin-2 is a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. Neutrophils migrate in response to increasing concentrations of chemotactic factors released from inflammatory sites. From the culture fluid of a human T-cell leukemia cell line (SKW-3), Yamagoe et al. (1996) isolated a 16-kD secreted protein with neutrophil chemotactic activity and designated it LECT2. They purified the protein and determined the amino acid sequence.

Mouse Leukocyte cell-derived chemotaxin-2 (LECT2) ELISA Kit employs a two-site sandwich ELISA to quantitate LECT2 in samples. An antibody specific for LECT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLECT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LECT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LECT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Leukocyte cell-derived chemotaxin-2 (LECT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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