Lefty proteins are signaling ligands that act in opposition to the NODAL protein. During vertebrate development they regulate the degree of left-right asymmetry by controlling the spatiotemporal influence of the NODAL protein.It is involved in embryogenesis and left-right patterning. There are many differences between the left and right sides, including heart and lung positioning. Mutations in these genes cause incorrect positioning of these organs (e.g., situs inversus). Lefty1 in the ventral midline prevents the Cerebrus (paracrine factor or "Caronte") signal from passing to the right side of the embryo.The role of Lefty1 is to restrict the expression of Lefty2 and Nodal to the left side, and that Lefty2 or Nodal encode a signal for 'leftness.' Lefty2 serves as a feedback inhibitor to restrict the range of nodal signaling during establishment of the left-right axis.

Mouse Left-right determination factor 2 (LEFTY2) ELISA Kit employs a two-site sandwich ELISA to quantitate LEFTY2 in samples. An antibody specific for LEFTY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEFTY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEFTY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEFTY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Left-right determination factor 2 (LEFTY2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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