Leptin receptor functions as a receptor for the fat cell-specific hormone leptin. LEP-R has also been designated as CD295. After co-discovering the Leptin gene with Jeffrey Friedman et al. in 1994, which involved a reverse genetic/positional cloning strategy to clone ob and db, Rudolph Leibel, working with collaborators at Millennium Pharmaceuticals and colleague Streamson Chua, confirmed cloning of the leptin receptor by demonstrating that an apparent leptin receptor cloned from a choroid plexus library using leptin as ligand, mapped to a physical map that included db and fa. The leptin hormone regulates adipose-tissue mass through hypothalamus effects on fullness and energy use. It acts through the leptin receptor (LEP-R), a single-transmembrane-domain receptor of the cytokine receptor family.

Mouse Leptin receptor (LR/Ob-R) ELISA Kit employs a two-site sandwich ELISA to quantitate LEPR in samples. An antibody specific for LEPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEPR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Leptin receptor (LR/Ob-R) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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