LIMS1 is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.While ILK depletion reduced AKT phosphorylation on ser473, PINCH1 depletion reduced AKT phosphorylation on both ser473 and thr308. PINCH1 also regulated ILK protein levels.

Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit employs a two-site sandwich ELISA to quantitate LIMS1 in samples. An antibody specific for LIMS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIMS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIMS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIMS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-lim-and-senescent-cell-antigen-like-containing-domain-protein-1-lims1-elisa-kit-kte71175/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.