The protein encoded by MED19 is a subunit of the Mediator complex, which binds to gene-specific regulatory factors and provides support for the basal RNA polymerase II transcription machinery. MED19 has been implicated in the growth of several types of cancer, and inhibition of its expression inhibits the growth and spread of these cancers. Two transcript variants encoding different isoforms have been found for MED19.

Mouse Mediator of RNA polymerase II transcription subunit 19 (MED19) ELISA Kit employs a two-site sandwich ELISA to quantitate MED19 in samples. An antibody specific for MED19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Mediator of RNA polymerase II transcription subunit 19 (MED19) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-mediator-of-rna-polymerase-ii-transcription-subunit-19-med19-elisa-kit-kte71067/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.