Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3-prime position. The products of these reactions serve as second messengers in growth signaling pathways. The kinase itself is made up of a catalytic subunit of molecular mass 110 kD (PIK3CA) and a regulatory subunit of either 85 kD, 55 kD, or 50 kD.Both p85-alpha and p85-beta bound Xbp1s and increased its nuclear translocation, and it appeared that the p110 PI3K catalytic subunit and Xbp1s competed for binding of these regulatory subunits. p85-alpha and p85-beta formed an inactive dimer that was disrupted by insulin in a time-dependent manner, which promoted their association with Xbp1s.

Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3R2 in samples. An antibody specific for PIK3R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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