Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control. Northern blot analysis demonstrated expression of an approximately 3.5-kb PLD2 transcript in various tissues including heart, placenta, pancreas, spleen, thymus, prostate, uterus, brain, lung, kidney, small intestine, and colon. Expression was relatively low in liver and skeletal muscle. An additional 3.8-kb transcript was expressed in peripheral blood leukocytes.

Mouse Phospholipase D2 (PLD2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLD2 in samples. An antibody specific for PLD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Phospholipase D2 (PLD2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.