PLOD2 is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. The similarity is even higher in the C-terminal region of the polypeptides. The PLOD2 gene contains 9 cysteines conserved in PLOD, in addition to conserved histidines in the putative active site.

Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLOD2 in samples. An antibody specific for PLOD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLOD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLOD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLOD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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