TMPRSS6 shares 35% sequence identity with human matriptase/ST14 and spinesin/TMPRSS5, and 84.3% identity with mouse Tmprss6. It contains structural features characteristic of type II serine proteinases, including a short hydrophobic domain, a type II transmembrane sequence, 2 CUB domains, 3 LDL receptor repeats, and a C-terminal catalytic domain with structural hallmarks of serine proteinases. A potential phosphorylation domain is present at the cytoplasmic tail. Sequence alignment of the catalytic domains of TMPRSS6 and other type II serine proteinase family members permitted the identification of several conserved domains. Northern blot analysis of multiple human tissues revealed expression of a 3.5-kb TMPRSS6 transcript exclusively in fetal and adult liver.

Mouse Transmembrane protease serine 6 (TMPRSS6) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS6 in samples. An antibody specific for TMPRSS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Transmembrane protease serine 6 (TMPRSS6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.